Methods for Producing Polypeptides in Protease-Deficient Mutants of Trichoderma

摘要

The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

主权项

1. An isolated mutant of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and a first aspartic protease gene, wherein the first aspartic protease gene is modified rendering the mutant strain at least 95% deficient in the production of the first aspartic protease compared to the parent Trichoderma strain when cultivated under identical conditions;
wherein the first aspartic protease is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 4 or amino acids 21 to 407 of SEQ ID NO: 4; and (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 3 or nucleotides 61 to 1299 of SEQ ID NO: 3, or the cDNA thereof; and wherein the polypeptide is native or foreign to the parent Trichoderma strain.