| 摘要 |
<p>Disclosed is a method for storing a cartilage-cell-adhering porous body in an airtight state over a long term of at shortest 14 days. The method comprises suspending isolated cartilage cells into atelocollagen at an administration cell concentration of 1.0 × 10 7 to 1.0 × 10 8 cells/ml to prepare a cell suspension, seeding the cell suspension to a biocompatible porous body, gelatinizing the cell suspension on the porous body to yield the cartilage-cell-adhering porous body, and keeping the cartilage-cell-adhering porous body at a temperature of 26 to 37°C in a medium having an amount of 1 mL or more per 1.0 × 10 5 cells that are administrated living cells out of the cartilage cells, containing insulin, a fibroblast growth factor, and a 5% or more serum, while the porous body is shaken to set the shaking speed of the medium in the range of 0 to 2.8 cm/s.</p> |
