| 摘要 |
<p>This invention describes a treatment for psoriasis (a skin disorder) with the keratinase enzyme based nanoformulation. Keratinase enzyme (EC 3.4.99.11) from the fungus Aspergillus parasiticus was purified to homogeneity by conventional techniques and used for making nanoformulation with the silver nanoparticles. The silver nanoparticles were biologically synthesized from the fungus Fusarium oxysporum. The keratinase nanoformulation was made by mixing the purified enzyme with silver nanoparticles and this nanoformulation is named as 'Nanozyme-K'. It was found that the silver nanoparticles did not have any inhibitory effect on the purified keratinase activity and in fact it improved the storage stability of the nanoformulation. Psoriatic samples were collected from patients and the psoriatic proteins were examined by SDS polyacrylamide gel electrophoresis. It was confirmed by MALDI-MS analysis that all the psoriatic proteins belong to keratins of various types. The Nanozyme-K effectively digested the keratins of psoriatic scales which were confirmed by SDS-PAGE analysis. It was found that the keratinase nanoformulation j degraded the psoriatic keratins completely and keratin bands were not observed after digestion in SDS-PAGE analysis. The efficacy of the Nanozyme-K was tested out on dermatitis induced BALB/c mice. About 36% reduction in ear swelling was observed after Nanozyme-K treatment. Thus, it confirms the Nanozyme-K possesses good antipsoriatic activity. As it is a gentle, non invasive technique it can be recommended for psoriatic patients even on long term basis without any possible side effects. Moreover, as it is a topical application, it will not have any possible adverse effect(s) like most of the drugs recommended.</p> |
