| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for determining the amount of dihydrotestosterone (DHT) in a body fluid sample using mass spectrometry.SOLUTION: The method comprises: ionizing underivatized DHT, purified by solid phase extraction (SPE) from the body fluid sample under conditions suitable to produce one or more ions detectable by mass spectrometry, where the ions comprise one or more ions selected from the group consisting of ions with a mass/charge ratio of 291.10±0.50, 255.20±0.50 and 79.20±0.50; and determining the amount of one or more ions by mass spectrometry, and using the amount determined to calculate the amount of underivatized DHT in the body fluid sample. |
