| 主权项 |
1. A method for detecting HIV-1 in a saliva sample at early stages of infection, comprising the steps of:
(A) providing glycan immobilized metal nanoparticles which can recognize HIV-1 and diluting the saliva sample to half of the concentration; (B) contacting the glycan immobilized metal nanoparticles with the saliva sample and a mixture is obtained, wherein a volume ratio of the glycan immobilized metal nanoparticles to the saliva sample is 1:49, the HIV-1 in the saliva sample is approximately 0.1 to 10 copies/ml; (C) concentrating the mixture; and (D) determining HIV-1 in the concentrated mixture by a detecting method; wherein each of the glycan immobilized metal nanoparticles includes a ligand-conjugate, the ligand-conjugate has a linker compound, the linker compound has a formula (1): wherein a, b, c, d, m1, m2 and m3 are independently an integer from 0 to 6, each of the aromatic amino groups of the linker compound is connected to a glycan with a reducing terminal end, the sulfur atom of the linker compound is bound to a metal nanoparticle, and the glycan is capable of specifically binding to HIV-1. |
