CYCLOPENTANE-PEPTIDE NUCLEIC ACIDS FOR QUALITATIVE AND QUANTITATIVE DETECTION OF NUCLEIC ACIDS

摘要

The invention concerns methods for detecting a nucleic acid of interest in a solution comprising (a) contacting a solution suspected of containing the nucleic acid of interest with a PNA capture probe and a PNA reporter probe; wherein (i) the PNA capture probe comprises at least two trans-cyclopentanes; (ii) the PNA reporter probe comprises at least six biotin groups; (iii) the PNA capture probe bound to a surface; and (iv) the PNA capture probe and the PNA reporter probe each comprise a nucleobase sequence that is complementary to different non-overlapping portions of the nucleic acid of interest; (b) detecting the presence of the PNA capture probe and the PNA reporter probe bound to the surface; wherein the nucleic acid of interest is detected when 1-1000 molecules of the nucleic acid of interest are present in the solution being tested.

主权项

1. A method of detecting a nucleic acid of interest in a solution, said method comprising
(a) contacting a solution suspected of containing said nucleic acid of interest with a PNA capture probe and a PNA reporter probe; wherein
(i) said PNA capture probe comprises at least two trans-cyclopentanes;(ii) said PNA reporter probe comprises at least six biotin groups;(iii) said PNA capture probe bound to a surface; and(iv) said PNA capture probe and said PNA reporter probe each comprise a nucleobase sequence that is complementary to different non-overlapping portions of said nucleic acid of interest; (b) detecting the presence of said PNA capture probe and said PNA reporter probe bound to said surface; wherein said nucleic acid of interest is detected when 1-1000 molecules of said nucleic acid of interest are present in the solution being tested.