Yeast Organism Producing Isobutanol at a High Yield

摘要

The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

主权项

1. A recombinant yeast microorganism for producing an acetolactate intermediate, wherein the recombinant yeast microorganism has been engineered to:
(a) express a heterologous acetolactate synthase (ALS); (b) disrupt, mutate, or delete one or more endogenous pyruvate decarboxylase (PDC) genes, wherein said recombinant yeast microorganism has reduced endogenous PDC activity as compared to the corresponding yeast microorganism that has not been engineered to have reduced endogenous PDC activity; and (c) disrupt, mutate, or delete one or more endogenous glycerol-3-phosphate dehydrogenase (GPD) genes, wherein said recombinant yeast microorganism has reduced endogenous GPD activity as compared to the corresponding yeast microorganism that has not been engineered to have reduced endogenous GPD activity;and wherein said heterologous ALS catalyzes the conversion of pyruvate to said acetolactate intermediate within said recombinant yeast microorganism.