| 摘要 |
The present disclosure describes a method of adapter ligation to the ends of fragmented double-stranded DNA molecules. This novel method overcomes the necessity to add a phosphate group to the 5' ends of DNA fragments. Instead, the 5' terminal bases that are damaged as a result of physical fragmentation of the DNA, are removed. By removal of the damaged base, a ligation compatible base with a 5' phosphate is exposed and adapter ligation efficiency is restored, leading to a significant increase in library yield and the ability to construct libraries from reduced input DNA quantities. |
