发明名称 |
A process of obtaining ethanol without glucoamylase using pseudomonas saccharophila G4-amylase and variants thereof |
摘要 |
Pseudomonas saccharophila G4-forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S . cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity. |
申请公布号 |
EP2554666(B1) |
申请公布日期 |
2015.08.05 |
申请号 |
EP20120190129 |
申请日期 |
2008.12.30 |
申请人 |
DANISCO US INC. |
发明人 |
SHAW, ANDREW;CHIN, REGINA;KRAGH, KARSTEN MATTHIAS;KELLET-SMITH, ANJA HEMMINGSEN |
分类号 |
C12N9/28;A21D8/04;A23L1/09;C12N9/24 |
主分类号 |
C12N9/28 |
代理机构 |
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代理人 |
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