Stirred Tank Bioreactor

摘要

The present invention is a disposable, presterilized bioreactor formed of molded plastic, having a top and body attached to each other. One or more ports are formed in the top, side and bottom of the housing. The one or more ports that are below the liquid/air interface level may be used as sampling ports, access ports for probes or drains or supply ports for liquids or gases. The bioreactor provides a direct retrofit for the existing glass or steel assembly that utilizes the existing support structures and controls. The molded design overcomes issues of discontinuity, dead spots and the like due to its fixed dimensions that are built in by the molding process. Reproducible probe and other equipment locations are guaranteed through the use of the molded port features.

主权项

1) A bioreactor for culturing, fermenting or processing a biomass comprising: a presterilized, disposable housing made of a rigid plastic, said housing having a top and a body releasably sealed to each other, wherein the top and body are sealed to each other by a gasket interposed between the top and body and a mechanical means for releasably holding the top and body together, the body having an interior space, a first set of one or more access ports formed in the top of the housing and in fluid communication with the interior of the housing, a second set of one or more access ports formed in the body and bottom of the housing and in fluid communication with the interior of the housing wherein at least one access port of the second set being a valved port in the bottom of the housing forming an outlet for liquid in the housing, wherein the valved port further allows for the entrance of gases into the housing through the port by the valve having a first position to allow liquid flow, a second position to allow gas flow and a third position to allow neither liquid nor gas to flow through the valve and at least one access port of the second set being formed in the body of the housing so as to allow for the introduction of liquid, probes or samplers into the interior of the body, the one or more access ports having a cap to isolate the interior space of the body from the environment.