Method of arraying ferritin

摘要

A method of selectively arraying ferritin and inorganic particles on a silicon oxide substrate having a chromium, niobium or tungsten portion. An aspect of the method includes steps of: preparing a solution which contains ferritin modified at an N-terminal part of a subunit with a peptide set out in SEQ ID NO: 1, and a nonionic surfactant; and a binding step of bringing the solution in contact with the silicon oxide substrate to selectively array peptide-modified ferritin to the chromium, niobium or, tungsten portion. Another aspect of the method includes selectively arraying ferritin modified with the peptide set out in SEQ ID NO: 1, and the inorganic particles contained in ferritin at the chromium, niobium, or tungsten portion by removing the solution.

主权项

1. A method of arraying ferritin, the method comprising steps of:
a preparing step of preparing a solution which contains-ferritin modified at an N-terminal of a subunit with a peptide set out in SEQ ID NO: 1, wherein a serine residue at the N-terminal of the subunit is bonded to an alanine residue of the peptide of SEQ ID NO: 1, and from 0.01 v/v % to 10 v/v % of a nonionic surfactant, wherein the solution has a pH falling within the range of from 7.4 to 8.2; and a binding step of bringing the solution in contact with a silicon oxide substrate having a metal portion of one metal selected from chromium, niobium, and tungsten formed on a part of the surface so as to selectively bind the ferritin to the metal portion.