Low affinity FCGR deficient mice

摘要

Genetically modified non-human animals and methods and compositions for making and using them are provided, wherein the genetic modification comprises a deletion of the endogenous low affinity FcγR locus, and wherein the mouse is capable of expressing a functional FcRγ-chain. Genetically modified mice are described, including mice that express low affinity human FcγR genes from the endogenous FcγR locus, and wherein the mice comprise a functional FcRγ-chain. Genetically modified mice that express up to five low affinity human FcγR genes on accessory cells of the host immune system are provided.

主权项

1. A transgenic mouse whose genome comprises a homozygous disruption in an endogenous FcγRIIB α-chain gene, a homozygous disruption in an endogenous FcγRIV α-chain gene, a homozygous disruption in an endogenous FcγRIII α-chain gene, and a nucleic acid sequence encoding a human CD20 gene, wherein the mouse specifically expresses functional CD20 on B cells.