Method of transfection and compositions therefor

摘要

The present invention relates to the targeted delivery of molecules to cells expressing toll-like receptors (TLRs). Aspects of the invention provide compounds comprising a positively charged group linked to a TLR ligand. These compounds are useful for in vitro and in vivo methods of transfection of TLR-expressing cells. Other aspects of the invention relate to the use of such compounds for repression of gene expression and DNA vaccination approaches.

主权项

1. A positively charged compound comprising a positively charged group linked to at least one TLR-2 or TLR-6 ligand, wherein the positively charged group comprises a branched peptide comprising at least four positively charged amino acid residues, wherein the branched peptide comprises:wherein
each X is independently a lysine residue, an arginine residue or a histidine residue.