| 摘要 |
FIELD: biotechnology.SUBSTANCE: method comprises growing microbial mass in the fermentor in the amount of 1200 litres for 7 days. Separation and destruction of the biomass on the module-separator. Separating the precipitate by centrifugation at 10,000 g for 15 minutes. The resulting supernatant is centrifuged at 50,000 g for 55 min, and the supernatant is discarded. To the resulting precipitate, the distilled water is poured in the same volume, the precipitate is suspended in water 3-4 times. After that it is applied to the chromatographic column with silica gel based on 200 optical units in the sample of application at a wavelength of 280 nm per 100 ml of silica gel. After elution, the samples are collected with the ratio of the optical density of the solution at a wavelength of 280 nm to the optical density of the solution at a wavelength of 570 nm (D280/D570) of less than 2.5.EFFECT: invention enables to obtain a high yield of bacteriorhodopsin, including through the use of a strain of Halobacterium salinarum RNCIM B-11850.1 tbl, 1 ex |
