MULTIPLEXED MICROCOLUMN DEVICES AND PROCESSES FOR SELECTION OF NUCLEIC ACID APTAMERS

摘要

The present invention relates to a microcolumn device for selecting nucleic acid aptamers for single and multiple target molecules, as well as a method for making the microcolumn device. The present invention also relates to a system for selecting nucleic acid aptamers for single and multiple target molecules. The present invention further relates to methods of using the microcolumn device for selecting nucleic acid aptamers for multiple target molecules. Kits that include one or more microcolumn device and/or system of the present invention are also disclosed.

主权项

1. A microcolumn device for selecting nucleic acid aptamers for single and multiple target molecules, said microcolumn device comprising:
a body comprising an inlet end, an outlet end, a recessed portion extending into the body from the outlet end and forming a compartment for housing a frit, and a microfluidic channel disposed between the inlet end and the recessed portion; a frit disposed in the recessed portion; and a pair of adapters comprising an inlet adapter bonded to the inlet end of the body and an outlet adapter bonded to the outlet end of the body, wherein each adapter includes a hollow passageway for passing a liquid sample there through, and wherein each adapter includes a connector region for effectuating serial fluid communication of a plurality of microcolumn devices.