Composition comprising ligustroflavone, rhoifolin and hyperin and its pharmaceutical application

摘要

Disclosed is a composition comprising ligustroflavone, rhoifolin and hyperin, which is prepared according to rational weight ratio: 40% to 80% ligustroflavone, 5% to 45% rhoifolin and 1% to 40% hyperin. The composition can be used as a neuraminidase inhibitor for preventing and treating influenza, and can be formulated into pharmaceutically acceptable dosage forms.

主权项

1. An extraction method of a composition comprising ligustroflavone, rhoifolin and hyperin from a turpinia montana leaf comprising steps:
taking the turpinia montana leaf; adding 30%˜90% ethanol which is 5 to 15 times the amount of the leaf; conducting reflux extraction for 1˜3 times, with 1˜3 hours each time, and then filtering to get filtrate; mixing the filtrates gotten from each time of the reflux extraction and recovering the ethanol to get water solution; eluting the water solution through a macroporous resin column, collecting elution part, reducing pressure, concentrating and drying the elution part, get mixed total glycosides; separating the mixed total glycosides by column chromatography to get liquid ligustroflavone, liquid rhoifolin and liquid hyperin respectively, crystallizing get the pure ligustroflavone, the pure rhoifolin and the pure hyperin.