发明名称 |
METHOD FOR PRODUCING CELL HAVING NUCLEIC ACID INTRODUCED THEREIN |
摘要 |
A method for producing a cell having nucleic acid introduced therein and having low cytotoxicity is useful in primary cells and slowly dividing cells, for which nucleic acid introduction is difficult with conventional techniques. A method for introducing nucleic acid into a cell includes the steps of mixing a nucleic acid with a temperature sensitive material at a temperature lower than the cloud point of the temperature sensitive material, the temperature sensitive material being formed by adding 2-amino-2-hydroxymethyl-1,3-propanediol to a temperature-sensitive polymer material having 2-N,N-dimethylaminoethyl methacrylate and/or a derivative thereof as the main polymer component, flow coating a culture vessel with the resulting mixed liquid, and culturing a cell suspension provided to the culture vessel at a temperature higher than the cloud point. |
申请公布号 |
EP2799544(A4) |
申请公布日期 |
2015.07.15 |
申请号 |
EP20120863681 |
申请日期 |
2012.12.26 |
申请人 |
NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER;BRIDGESTONE CORPORATION |
发明人 |
NAKAYAMA, YASUHIDE;IWAI, RYOSUKE;NEMOTO, YASUSHI |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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