发明名称 |
Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria |
摘要 |
A high flux of metabolites from pyruvate to 2,3-butanediol in Lactobacillus plantarum was achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products. |
申请公布号 |
US9080179(B2) |
申请公布日期 |
2015.07.14 |
申请号 |
US201313872873 |
申请日期 |
2013.04.29 |
申请人 |
BUTAMAX ADVANCED BIOFUELS LLC |
发明人 |
Paul Brian James |
分类号 |
C12N15/74;C12N9/04;C12P7/16;C12P7/18;C12P7/26 |
主分类号 |
C12N15/74 |
代理机构 |
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代理人 |
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主权项 |
1. A recombinant lactic acid bacterial cell comprising at least one gene encoding a heterologous polypeptide having butanediol dehydrogenase activity wherein the bacterial cell is substantially free of lactate dehydrogenase activity and wherein the cell produces 2,3-butanediol, and wherein the cell comprises a 2-butanol or 2-butanone biosynthetic pathway. |
地址 |
DE US |