| 摘要 |
The invention provides transgenic Escherichia coli cells comprising a mutation in cydAB gene, and/or cyoABCD gene, and/or cbdAB gene, and/or ygiN gene, wherein the mutation reduces (preferably, but not necessarily, by 100%) the cytochrome oxydase activity of a protein encoded by cydAB gene, and/or cyoABCD gene, and/or cbdAB gene, and/or ygiN gene. In a preferred embodiment, the mutation is a deletion of the cydAB gene, cyoABCD gene, and cbdAB gene. In another embodiment, the mutation is a deletion of the cydAB gene, cyoABCD gene, cbdAB gene, and ygiN gene. In another embodiment, the transgenic Escherichia coli cell, which comprises a deletion of cydAB gene, and/or cyoABCD gene, and/or cbdAB gene, and/or ygiN gene a) has substantially the same level of growth in oxic conditions as the level of growth in anoxic conditions of Escherichia coli that lacks said deletion of cydAB gene, and/or cyoABCD gene, and/or cbdAB gene, and/or ygiN gene, and b) is capable of converting glucose to D-lactate and/or amino acid and/or 2,3-butanediol (2,3-BDO) under one or both of oxic conditions and anoxic conditions. |
| 主权项 |
1. A transgenic Escherichia coli cell comprising an additional copy of Escherichia coli alanine dehydrogenase (DadA) gene, and a deletion in the endogenous cydA gene (SEQ ID NO: 1), cydB gene (SEQ ID NO: 2), cyoA gene (SEQ ID NO: 3), cyoB gene (SEQ ID NO: 4), cyoC gene (SEQ ID NO: 5), cyoD gene (SEQ ID NO: 6), cbdA gene (SEQ ID NO: 7), cbdB gene (SEQ ID NO: 8), ygiN gene (SEQ ID NO: 9), and ldhA gene, wherein said transgenic Escherichia coli cell
a) has substantially the same level of growth in oxic conditions as the level of growth in anoxic conditions of the corresponding Escherichia coli cell that has no deletion of the endogenous cydA gene, endogenous cydB gene, endogenous cyoA gene, endogenous cyoB gene, endogenous cyoC gene, endogenous cyoD gene, endogenous cbdA gene, endogenous cbdB gene, and endogenous ygiN gene, b) is capable of converting glucose to D-lactate under oxic conditions and/or anoxic conditions, c) has substantially the same level of growth as said corresponding Escherichia coli in M9 minimal essential media that
(i) comprises a carbon source consisting of glucose, and(ii) lacks amino acids Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and Val, and d) is capable of producing an increased level of alanine compared to the corresponding Escherichia coli cell that comprises the endogenous ldhA gene and that does not comprise a heterologous DNA sequence encoding DadA. |
