Method of storing plant embryos

摘要

Methods for obtaining genetic material from plant embryos while preserving their viability are provided. In the methods, preservation of viability may be maintained by suspending the embryos in an aqueous solution surrounded by an oil matrix. Genetic material may be obtained from an aliquot of the aqueous solution and may be used directly for molecular analysis, or whole genome amplification may be performed prior to molecular analysis.

主权项

1. A method of storing one or more plant embryos comprising placing the one or more plant embryos in an aqueous solution surrounded by a matrix of two oils, wherein one of the two oils is more dense than the aqueous solution and one of the two oils is less dense than the aqueous solution, wherein the aqueous solution is surrounded by the oil that is more dense than the aqueous solution and the oil that is less dense than the aqueous solution.