发明名称 |
METHOD AND APPARATUS FOR DETERMINING DEGREE OF STRATIFICATION AND/OR DIFFERENTIATION |
摘要 |
A method for easily and non-invasively determining a degree of stratification and/or differentiation of cultured cells, and an apparatus for determining a degree of stratification and/or differentiation of cultured cells are provided. The invention relates to a method for determining a degree of stratification and/or differentiation of cultured cells, including: (a) a harvesting step of harvesting a culture solution of the cultured cells; (b) an analytical step of analyzing at least one type of a metabolite of the pentose phosphate pathway and/or the TCA cycle in the culture solution; and (c) a determination step of referring to a database of correlations between prospectively-obtained degrees of stratification and/or differentiation of the cultured cells and analytical results of metabolites, for analytical results obtained in the analytical step, to determine the degree of stratification and/or differentiation of the cultured cells. |
申请公布号 |
US2015192568(A1) |
申请公布日期 |
2015.07.09 |
申请号 |
US201314419185 |
申请日期 |
2013.07.17 |
申请人 |
Hitachi, Ltd. |
发明人 |
Shibuya Keisuke;Haga Ryoichi;Namba Masaru;Nakajima Ryota |
分类号 |
G01N33/50 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
1. A method for determining a degree of stratification and/or differentiation of cultured cells, comprising:
(a) a harvesting step of harvesting a culture solution of the cultured cells; (b) an analytical step of analyzing at least one type of a metabolite of the pentose phosphate pathway and/or the TCA cycle in the culture solution; and (c) a determination step of referring to a database of correlations between prospectively-obtained degrees of stratification and/or differentiation of the cultured cells and analytical results of metabolites, for analytical results obtained in the analytical step, to determine the degree of stratification and/or differentiation of the cultured cells. |
地址 |
Tokyo JP |