Inhibitors of atypical protein kinase C and their use in treating hedgehog pathway-dependent cancers

摘要

Methods and compositions are provided for modulating Hedgehog (Hh) pathway signaling in a cell. Aspects of the methods include methods for inhibiting Hh pathway-promoted cancer proliferation and/or metastasis that is promoted by Hh pathway signaling, methods for treating cancers promoted by Hh pathway signaling, and methods for screening candidate agents for the ability to treat a cancer promoted by Hh pathway signaling. In addition, reagents and kits thereof that find use in practicing the subject methods are provided.

主权项

1. A method of inhibiting Hedgehog (Hh) pathway-promoted cancer cell proliferation, comprising:
determining Gli activity in a cancer cell to identify the cancer cell as a Hh-induced cancer cell, wherein Gli activity is increased in the Hh-induced cancer cell and aPKC iota activates Gli; contacting the Hh-induced cancer cell with an aPKC iota antagonist in an amount effective to inhibit Hh pathway-induced proliferation of the cell, wherein the aPKC iota antagonist consists essentially of SEQ ID NO:4.