Internal focus reference beads for imaging cytometry

摘要

The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological samples. More particularly, the invention provides systems and methods for internal calibration and focus reference for cytometry imaging.

主权项

1. A method for determining the presence or concentration of target cells in a sample, comprising:
mixing a sample to be tested for the presence or concentration of target cells with a pre-determined amount of micro-particles, wherein the target cells are capable of fluorescent emission at a first detection wavelength upon excitation at a first excitation wavelength, and wherein the microparticles are capable of fluorescent emission at a second detection wavelength upon excitation at a second excitation wavelength; loading the sample into a sample chamber of a fluorescent imaging system; directing a light beam at the sample, wherein the light beam comprising the second excitation wavelength; acquiring a fluorescent image of the sample at the second detection wavelength; calibrating the fluorescent imaging system with the fluorescent image acquired at the second detection wavelength; directing a second light beam at the sample, wherein the second light beam comprising the first excitation wavelength; acquiring a fluorescent image of the sample at the first detection wavelength; and determining the presence or concentration of the target cells in the sample.