Compositions and Methods for Tissue Storage

摘要

The disclosure provides methods, and related compositions, for treating tissue with trehalose, where treatment reduces water loss during subsequent storage, for example, frozen storage.

主权项

1. A method for processing a first composition that comprises muscle fibers, the method comprising:
Step a. Contacting a solution of a first concentration of trehalose (Solution A) to a first composition that comprises muscle fibers; Step b. Incubating the first composition that comprises muscle fibers, for a pre-determined time, at a first temperature that is above freezing, in the presence of the Solution A; and, Step c. Reducing or eliminating the presence of the Solution A; wherein the concentration of trehalose in the Solution A is capable of reducing weight loss of the first composition and is also capable of reducing water loss from the first composition, as determinable by subjecting to at least three cycles of freezing and thawing, as compared to a second control composition that comprises muscle fibers that was not incubated in the presence of trehalose, where the second control composition is subjected to the at least three cycles of freezing and thawing.