SYSTEMS, METHODS, AND APPARATUS FOR MULTICHANNEL IMAGING OF FLUORESCENT SOURCES IN REAL TIME

摘要

Presented herein is a multichannel imaging system capable of detecting and distinguishing multiple fluorescent light sources simultaneously. Also described herein are methods of using the system to image disease or cellular abnormalities, e.g., for diagnostic and/or intraoperative purposes.

主权项

1. A method for optical imaging of a region within a subject, the method comprising:
(a) administering to the subject two or more different probe species each comprising a fluorescent reporter; (b) directing excitation light into the subject, thereby exciting the fluorescent reporters; (c) simultaneously detecting fluorescent light of different wavelengths, the detected fluorescent light having been emitted by the fluorescent reporters of the probe species in the subject as a result of excitation by the excitation light so as to discriminate between signals received from each probe species; and (d) processing signals corresponding to the detected fluorescent light to provide one or more images (e.g. a real-time video stream) of the region within the subject.