发明名称 |
METHOD FOR SIZED-BASED CELL SEPARATION USING SPINNING MEMBRANE FILTRATION |
摘要 |
A method is provided for separating a suspension of cellular material comprising at least two differently-sized cell types using a spinning membrane separator. The method comprises selecting the cell type to be separated by passing through the membrane; determining a concentration of the selected cell type in the suspension; selecting an inlet flow rate for the suspension; selecting a rotational speed for the spinning membrane separator related to one or more of the concentration and relative size of the selected cell type in the suspension; rotating the spinning membrane separator at the selected rotational speed so that the selected cell type tends to migrate to regions of the shear field adjacent the porous membrane; and flowing the suspension through the spinning membrane separator. |
申请公布号 |
US2015182682(A1) |
申请公布日期 |
2015.07.02 |
申请号 |
US201314140978 |
申请日期 |
2013.12.26 |
申请人 |
Wegener Christopher;Olson Bret;Min Kyungyoon |
发明人 |
Wegener Christopher;Olson Bret;Min Kyungyoon |
分类号 |
A61M1/34 |
主分类号 |
A61M1/34 |
代理机构 |
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代理人 |
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主权项 |
1. A method for separating a suspension of cellular material comprising at least two differently-sized cell types using a spinning membrane separator, the steps comprising:
a) selecting the cell type to be separated by passing through the membrane; b) determining a concentration of each cell type in the suspension; c) selecting an inlet flow rate for the suspension; d) selecting a rotational speed for the spinning membrane separator directly related to one or more of the concentration and relative size of the selected cell type in the suspension; e) rotating the spinning membrane separator at the selected rotational speed; and f) flowing the suspension through the spinning membrane separator. |
地址 |
Libertyville IL US |