Modified-immobilized enzymes of high tolerance to hydrophilic substrates in organic media

摘要

Disclosed are preparations of modified interfacial enzymes, particularly lipases and phospholipases, immobilized on a solid support, wherein the enzyme is surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products. The enzyme may be protected by being covalently bonded with lipid groups which coat the enzyme, or by being immobilized or embedded in a hydrophobic solid support. Also disclosed are processes for the preparation of the hydrophobically protected enzymes. The enzymes may be efficiently used in the preparation of biodiesel.

主权项

1. A modified active interfacial enzyme immobilized on a solid hydrophobic support, wherein said enzyme is a lipase, an esterase or a phospholipase and wherein said enzyme is coated with lipid epoxide groups that are covalently bonded to surface nucleophilic groups of said enzyme via their original epoxy group, and said modified enzyme is thereby surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products.