| 摘要 |
Discloses a proNGF mutant wherein the protease cleavage site R1SK3R4 is substituted at least at positions R1 and K3 corresponding to positions 101 and 103 of the human wildtype proNGF sequence (SEQ ID NO: 1) by any amino acid selected from a non-basic amino acid and Histidine, wherein the sequence is defined as in the complete specification, and related methods of preparing a biologically active human beta-NGF. |
