| 摘要 |
Provided is a cell culture substrate, wherein polymer (B) having a lower critical solution temperature contained in the substrate is an uncrosslinked copolymer of a monomer (a) that becomes a hydrophobic polymer in homopolymerization and a monomer (b or c or d) that becomes a hydrophilic polymer in homopolymerization, and the lower critical solution temperature of the obtained copolymer (B) can be controlled widely by the types and ratio of the two monomers, making it possible to easily detach the cultured cells from the culture substrate surface rapidly without use of protein hydrolase or the like and without damage to the cells, and collect the cells. This cell culture substrate contains a polymer (A) of a (meth)acrylic acid ester monomer (a), one or more types of inorganic material (C) selected from water-swellable clay minerals and silica, and a polymer (B) having a lower critical solution temperature and comprising a monomer (a) and a monomer (b or c or d). |
