ASSAY FOR IDENTIFYING COMPOUNDS WHICH AFFECT STABILITY OF MRNA

摘要

The present invention relates to an assay for the identification of biological active compounds, in particular to a reporter gene assay for the identification of compounds, which have an effect on mRNA stability. More particularly, the present invention relates to a reporter gene expression system and cell lines comprising said expression system. The invention further relates to compounds which destabilise mRNA.

主权项

1. A DNA expression vector comprising:
i) a first DNA sequence comprising the coding sequence for one or more protein having a detectable signal; ii) one or more 3′ UTR sequence and one or more expression control sequence operatively associated with said coding sequence, and iii) a heterologous instability sequence DNA inserted into said 3′ UTR sequence comprising a second DNA sequence corresponding to one or more mRNA instability sequence derived from one or more naturally occurring genes.