XYLOSE FERMENTING YEAST CONSTRUCTED USING A MODIFIED GENOME SHUFFLING METHOD

摘要

Disclosed is a method of providing a recombinant microorganism. The method comprises the steps of: (a) providing a hybrid microorganism comprising DNA from a host microorganism and a donor microorganism; and (b) fusing DNA extracted from a second microorganism into the hybrid microorganism to form the recombinant microorganism. Also disclosed are recombinant yeasts produced by the method of the present disclosure and a method of fermenting sugar using the recombinant yeast produced by the method of the present disclosure.

主权项

1. A method of providing a recombinant microorganism, said method comprising:
(a) providing a hybrid microorganism comprising whole genomic DNA from a host microorganism and a donor microorganism, wherein the hybrid microorganism is thus a microorganism having genomes from two different parent microorganism, the hybrid microorganism is produced by transferring the whole genome of the donor microorganism to the host microorganism by whole genome transformation; and the hybrid microorganism exhibits an enhanced activity as compared to its host microorganism and/or donor microorganism individually; and (b) fusing whole genomic DNA extracted from a second microorganism into the hybrid microorganism to form said recombinant microorganism.