发明名称 Magnetic aggregating and washing device for in vitro assays and methods of use thereof
摘要 Provided herein are magnetic aggregating and washing devices. Further provided herein are methods using magnetic aggregation to aggregate and wash sample molecules in an in vitro assay.
申请公布号 US9063044(B2) 申请公布日期 2015.06.23
申请号 US201313779465 申请日期 2013.02.27
申请人 PLEXBIO CO. LTD. 发明人 Kao Chien-Teng;Chung Yao-Kuang;Lee Pei-San
分类号 G01N33/543;G01N1/34;B01L9/00;G01N35/00 主分类号 G01N33/543
代理机构 Morrison & Foerster LLP 代理人 Morrison & Foerster LLP
主权项 1. A method for washing a sample in an assay, the method comprising: a) coupling a microtiter plate with a magnetic structure, wherein the microtiter plate sits on top of the magnetic structure,wherein the microtiter plate comprises a well comprising a sample and a plurality of magnetic beads,wherein the magnetic structure comprises a plurality of magnets each having a first pole and a second pole, the first pole and the second pole having opposite magnetic properties, andwherein a first magnet of the magnetic structure and a second magnet of the magnetic structure are oriented in the magnetic structure such that the first pole of the first magnet faces the first pole of the second magnet, the first magnet adjacent to the second magnet in the magnetic structure; b) rotating the microtiter plate such that the microtiter plate and the magnetic structure move together at a first speed for a first duration to distribute the plurality of magnetic beads in the well, wherein the rotation of the microtiter plate at the first speed produces a relative centrifugal force that exceeds the magnetic force between the magnetic beads in a well of the microtiter plate and the magnetic structure; and, c) rotating the microtiter plate such that the microtiter plate and the magnetic structure move together at a second speed for a second duration to aggregate a majority of the plurality of magnetic beads at a portion of the well, the second speed different from the first speed.
地址 Taipei TW