PAIR OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR DETECTION OF VIRUS OF IMMUNODEFICIENCY OF CATS AND METHOD OF DIAGNOSTICS OF VIRUS OF IMMUNODEFICIENCY OF CATS

摘要

FIELD: biotechnologies.SUBSTANCE: pair of primers has the following structure - PF: 5'-TGGAGAAGGGAATTTCAGATGGG-3' and PR: 5'-TTTGGGTCAAGTGCTACATATTGTGG-3'. The method includes performing of PCR with detection of obtained results by a horizontal electrophoresis method in 1.5% agarose gel. Amplification is performed in the following mode: total denaturation at 95°C for 5 min., denaturation cycle at 95°C for 20 or 60 sec., annealing at 56°C for 20 or 60 sec., elongation at 72°C for 40 or 60 sec. The cycle denaturation - annealing - elongation is repeated 35 times. The final elongation is performed at 72°C for 5 min. In case of existence of an amplificated fragment of nucleotide sequence with the length 366 p.n. the presence of FIV in a cat organism is obvious.EFFECT: invention allows to perform diagnostics of a virus immunodeficiency of cats with high efficiency.2 cl, 6 dwg, 3 tbl, 3 ex