| 摘要 |
FIELD: agriculture.SUBSTANCE: invention is a method of reproduction of carnation in vitro, comprising separating explants, sterilisation, planting to Murashige and Skoog nutrient medium supplemented with sucrose 30 g/l and growth regulators 1 mg/l 6-benzylaminopurine and 0.2 mg/l naphthylacetic acid, micropropagation by removal of shoots from explants, their rooting in the Murashige and Skoog nutrient medium and further rooting in the ground, characterised in that in the micropropagation the explants are rooted on agar Murashige and Skoog nutrient medium supplemented with Ribav-Extra 0.01-0.1 mg/l, and rooted under the conditions ex vitro in the ground consisting of a mixture of humus - 50%, lowland peat with black mould humus - 30% and sand 20%.EFFECT: invention enables to simplify the process of micropropagation.1 tbl |
