ASPIRATION-FREE WELL PLATE APPARATUS AND METHODS

摘要

A well plate includes a including a top portion, a bottom portion and a membrane disposed between the top portion and the bottom portion. The top portion defines a sample well in fluid communication with an opening defined by the membrane and in fluid communication with a reservoir defined by the bottom portion. The well plate is configured to be used in a centrifugation process of a test sample including a sample material and a wash liquid. The test sample configured to be received within the sample well and the reservoir. The membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the reservoir, through the membrane and can be captured within a collection chamber while the sample material remains within the reservoir.

主权项

1. A method, comprising:
disposing a test sample within a sample well of a well plate, the well plate including a top plate, a bottom plate and a membrane disposed between the top plate and the bottom plate, the test sample including a wash liquid and a sample material; centrifuging the test sample such that a centripetal acceleration associated with the centrifuging draws the sample material away from the membrane and opposes a binding of the sample material to the membrane, the membrane configured to filter the wash liquid from the test sample during the centrifuging such that the wash liquid can pass from a reservoir defined by the bottom plate in a direction that directly opposes the centripetal acceleration during centrifuging, and is captured within a collection chamber in fluid communication with the reservoir via the membrane, while the sample material remains within the reservoir.