REAGENT KIT, ASSAY METHOD, MICROFLUIDIC DEVICE, AND ASSAY APPARATUS

摘要

An assay method enhances reliability of assay of a target material by removing interfering influence of non-target materials through controlling the concentration of the target material in a sample. A reagent kit, a microfluidic device, and a test apparatus are disclosed. The method includes capturing a target material using a capturing agent which selectively binds the target material and determining measured values of reaction products of an enzyme which is activated by the target material, wherein enzyme reactions are conducted in the presence and absence of the capturing agent.

主权项

1. A method of determining a concentration of a target material in a sample, the method comprising:
mixing the sample with reagent 1, said reagent 1 comprising (i) an enzyme which is activated by the target material and (ii) at least one substrate of the enzyme, thereby causing the activated enzyme to act on the substrate to produce an enzyme reaction product 1; separately mixing the sample with reagent 2, said reagent 2 comprising (i) a same enzyme which is activated by the target material, (ii) same at least one substrate of the enzyme, and (iii) a capturing material specifically capturing the target material, thereby binding at least part of the target material to the capturing material and causing the activated enzyme to act on the substrate to produce an enzyme reaction product 2; measuring characteristics of the enzyme reaction product 1 and of the enzyme reaction product 2; obtaining a difference value between the measured characteristics of the enzyme reaction product 1 and the measured characteristics of the enzyme reaction product 2; and determining the concentration of the target material included in the sample using the calculated difference value.