Selective Nucleic Acid Fragment Recovery

摘要

The invention provides methods and kits for nucleic acid purification and fragment selection and recovery. By adjusting salt concentrations and/or pH of the binding buffers, only nucleic acid fragments with desired size ranges are able to reversibly and non-specifically bind to a solid surface in certain salt concentrations and/or pH conditions, and can be subsequently eluted and/or recovered from the solid surface in water and/or a low salt eluting buffer.

主权项

1. A method to selectively recover nucleic acid fragments with a desired size range comprising:
a) mixing target nucleic acids with a binding buffer having a selected pH and salt concentration to allow the nucleic acid fragments with the desired size range to be bound to a solid surface; b) applying the mixture from step a) to the solid surface so that the nucleic acid fragments with the desired size range reversibly and non-specifically bind to the solid surface; and c) selectively recovering the nucleic acid fragments with the desired size range by eluting the bound nucleic acid fragments from the solid surface with an eluting buffer.