Cassette containing growth medium

摘要

The invention features devices for capturing and culturing cells (e.g., microorganisms, cells containing microorganisms, or cells from eukaryotic cell cultures) and methods of using these devices. One device is a cassette containing growth media that may be employed in an automated rapid enumeration system. The cassette has, for example, been enhanced with features for controlling surface flatness, optical imaging, controlled dehydration of semi solid nutrient media, controlled air and particle exchange, and automated handling. Another device of the invention is a filtration funnel that may used to concentrate cells in a sample onto a membrane.

主权项

1. A cell culturing device comprising a housing that contains growth medium for microorganisms; a detachable, sealable lid comprising an optically clear window disposed to allow imaging of said growth medium; a substantially non-radiative, substantially non-reflective rim that covers the edge of said growth medium; and a fiducial mark comprising a radiative component,
wherein said device is configured to allow placement of a membrane over said growth medium so that said rim covers the edge of said membrane and any exposed growth medium.