Composition comprising array of nucleic acid primer sets

摘要

This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.

主权项

1. A composition of matter that comprises
an array of solid surfaces comprising a first discrete area and a second discrete area;
wherein said first discrete area and said second discrete area each comprises:
a first set of nucleic acid primers; anda second set of nucleic acid primers wherein
said nucleic acid primers are fixed or immobilized to said array;the nucleotide sequences in said first set of nucleic acid primers are different from the nucleotide sequences in said second set of nucleic acid primers;the nucleotide sequences of a first set of nucleic acid primers of a first discrete area and the nucleotide sequences of a first set of nucleic acid primers of a second discrete area differ from each other by at least one base;the nucleotide sequences of the second set of nucleic acid primers of a first discrete area and the nucleotide sequences of the second set of nucleic acid primers of a second discrete area are the same sequence, said sequence being complementary or identical to an inherent universal detection target (UDT) sequence, wherein the nucleotide sequences of said second set of nucleic acid primers of a first discrete area are identical to the nucleotide sequences of said second set of nucleic acid primers of a second discrete area; a nucleotide sequence in the first set of nucleic acid primers in the first discrete area is complementary to a first sequence in a first nucleic acid of interest and a nucleotide sequence in the second set of nucleic acid primers in the first discrete area is identical to a second sequence in the first nucleic acid of interest; and a nucleotide sequence in the first set of nucleic acid primers in the second discrete area is complementary to a first sequence in a second nucleic acid of interest and a nucleotide sequence in the second set of nucleic acid primers in the second discrete area is identical to a second sequence in the second nucleic acid of interest.