| 摘要 |
Provided is a device comprising an upper chamber, a middle chamber and a lower chamber, wherein the upper chamber is in communication with the middle chamber through a first pore, and the middle chamber is in communication with the lower chamber through a second pore, wherein the first pore and second pore are about 1 nm to about 100 nm in diameter, and are about 10 nm to about 1000 nm apart from each other, and wherein each of the chambers comprises an electrode for connecting to a power supply. Methods of using the device are also provided, in particular for sequencing a polynucleotide. |
| 主权项 |
1. A method for controlling the movement of a charged polymer through a pore, the method comprising the steps of:
a) providing a dual-pore, dual-amplifier device for controlling the movement of a charged polymer through a first and second pore simultaneously, the device comprising (i) an upper chamber, a middle chamber and a lower chamber, (ii) a first pore connecting the upper chamber and the middle chamber, and (iii) a second pore connecting the middle chamber and the lower chamber; wherein the device further comprises (iv) a power supply configured to provide a first voltage between the upper chamber and the middle chamber, and provide a second voltage between the middle chamber and the lower chamber, each voltage being independently adjustable, and wherein the device provides (v) dual-amplifier electronics configured for independent voltage control and current measurement at each pore, and (vi) wherein the first and second pores are configured so that the charged polymer is capable of simultaneously moving across both pores in either direction and in a controlled manner, b) loading a charged polymer in the upper chamber, middle chamber, or lower chamber of the device; and c) setting an initial first voltage and an initial second voltage so that at least a portion of the polymer moves through the first pore and the second pore; d) detecting the presence of the charged polymer in both pores simultaneously; and e) when the charged polymer is in both pores simultaneously, applying competing voltage to the first and second pore to control the movement of the charged polymer through both pores simultaneously by adjusting the first voltage, the second voltage, or both. |
