USE OF SUCROSE AS SUBSTRATE FOR FERMENTATIVE PRODUCTION OF 1,2-PROPANEDIOL

摘要

The present invention is relative to a method for producing 1,2-propanediol by fermentation, comprising: cultivating a microorganism producing 1,2-propanediol in an appropriate medium comprising a source of sucrose, and recovering the 1,2-propanediol being produced, wherein the microorganism is able to utilize sucrose as sole carbon source for the production of 1,2-propanediol. In a preferred aspect of the invention, the source of sucrose is obtained from plant biomass, and is in particular sugar cane juice.

主权项

1. A method for producing 1,2-propanediol by fermentation, comprising:
a) cultivating a strain of Escherichia coli producing 1,2-propanediol in an appropriate medium comprising at least a carbon source, and b) recovering the 1,2-propanediol being produced, wherein the strain of Escherichia coli: is genetically modified to introduce a non-phosphotransferase system (non-PTS) sucrose utilization system with the introduction of the genes cscBKAR wherein the gene cscB codes for a sucrose:proton symport transport system, the gene cscK codes for a fructokinase, the gene cscA codes for an invertase, the gene cscR codes for a sucrose specific receptor, andis able to utilize sucrose as sole carbon source for the production of 1,2-propanediol.