METHOD OF DETECTING AND GENOTYPING BACTERIA Pasteurella multocida OF CATTLE OF SEROGROUPS A, B, D, E, F IN MULTIPLEX POLYMERASE CHAIN REACTION

摘要

FIELD: biotechnology.SUBSTANCE: method comprises performing PCR with electrophoretic detection of results, the transfer of amplification product on the gel, and assessment of the reaction. At that the study of each sample is carried out in two reactions using primers. In the first reaction the bacterium Pasteurella multocida is detected and the serogroups A and D are genotyped, in the second reaction the serogroups B, E and F are genotyped. The method enables to identify and genotype the strains and isolates of bacterium Pasteurella multocida of five serogroups - A, B, D, E, and F in pure or mixed cultures, as well as directly in samples of biological material from animals. The method may be used in veterinary microbiology for diagnostics of pasteurellosis in farm animals.EFFECT: increase in the accuracy of diagnostics.4 tbl, 4 ex