Carrier for holding nucleic acid

摘要

A solid support for maintaining a uniform spot shape while improving the capacity for immobilization of nucleic acids upon spotting of nucleic acids onto a solid support is provided. A substrate has a base material, an electrostatic layer formed on the base material in order to electrostatically attract nucleic acids, and carboxyl groups formed on the electrostatic layer, wherein the ratio, (COO peak intensity)/(C—C peak intensity) in the C1s spectra obtained by X-ray photoelectron spectrometry (XPS) performed for the surface of the substrate ranges from 0.10 to 0.20. The solid support for immobilizing nucleic acids is produced by active esterification of carboxyl groups of the substrate.

主权项

1. A nucleic-acid-immobilized solid support for immobilizing a nucleic acid, prepared by active esterification of a carboxyl group of a substrate having a base material, an electrostatic layer formed on the base material to electrostatically attract a nucleic acid, and a carboxyl group formed on the electrostatic layer, wherein the ratio, (COO peak intensity)/(C—C peak intensity), in the C1s spectra obtained by X-ray photoelectron spectrometry (XPS) performed upon the surface of the substrate ranges from 0.10 to 0.20; and wherein a nucleic acid solution is spotted on the solid support to make a plurality of independent spots having said nucleic-acids.