Method of treating inflammation using human immunosuppressive protein

摘要

A method of treating inflammation by administering a therapeutically effective amount of a human immunosuppressant protein (HISP) to a subject is presented. The inventors have discovered a novel immunosuppressive protein purified from the supernatant of hNT cell culture. The immunosuppressant protein has a molecular weight of about 40-100 kDa, an isoelectric point of about 4.4, a net ionic charge and is capable of suppressing T-cell activation, T-cell proliferation and the production of IL-2. This protein can be used in treating inflammation, preventing graft rejection after transplantation, treating autoimmune diseases and suppressing allergic responses as well as other uses.

主权项

1. A method of reducing T-lymphocyte mediated inflammation in a subject comprising:
administering a therapeutically effective amount of a human immunosuppressant protein (HISP) to the subject, wherein the HISP protein is obtained by: a) growing a culture of hNT neuronal cells; b) obtaining supernatant from the hNT cell culture; c) concentrating the supernatant; d) exposing the supernatant to gel electrophoresis to produce isoelectric fractions; e) collecting a peak immunosuppressive active isoelectric fraction; and f) collecting the protein extract from the fraction; and wherein the HISP protein has an isoelectric point of about 4.8, a molecular weight of between about 40-100 kDa and exhibits at least one immunosuppressive activity selected from the group consisting of suppressed T-cell activation, suppressed T-cell proliferation, and suppressed production of IL-2 by T-cells.