| 主权项 |
1. A method of cryopreserving an implantable cell culture device, the device comprising:
a) a core comprising
(i) a cell line comprising an ARPE-19 cell genetically engineered to produce a therapeutically effective amount of one or more cytokines, neurotrophic factors, soluble receptors, anti-angiogenic antibodies and molecules, or biologically active molecules that are introduced into the ARPE-19 cell by an iterative transfection process, wherein the iterative transfection comprises one transfection, two transfections, or three transfections,(ii) a cell line comprising an ARPE-19 cell genetically engineered to produce a therapeutically effective amount of one or more cytokines, neurotrophic factors, soluble receptors, anti-angiogenic antibodies and molecules, or biologically active molecules that at least 10,000 ng/day/106 cells; or(iii) ARPE-19 cells genetically engineered to secrete a therapeutically effective amount one or more biologically active molecules, and b) a semi-permeable membrane surrounding the cell line in (i), the cell line in (ii), or the ARPE-19 cells in (iii), wherein the membrane permits the diffusion of the cytokines, neurotrophic factors, soluble receptors, anti-angiogenic antibodies and molecules, or biologically active molecules there through,the method comprising the steps of:
adding a cryoprotectant agent to the core of the device, placing the device in a cryogenic storage vial, freezing the devices under controlled rate freezing, and storing the device in dry ice (−70° C.), in a freezer (−80° C.), in vapor phase liquid nitrogen (−190° C.), or any combination thereof. |
