ASSAYS, METHODS AND APPARATUS FOR ASSESSING RNA DISRUPTION

摘要

Various embodiments are described herein related to an assay, method and apparatus for performing an RNA Disruption Assay (RDA) for cellular RNA optionally in response to a cytotoxic treatment such as chemotherapy and/or radiation treatment. The method comprises obtaining at least one electropherogram dataset corresponding to a unique biological sample comprising the cellular RNA at a time point, optionally during or after the treatment; determining values for features from at least two shifted regions of the at least one electropherogram dataset, the shifting being due to the treatment; and optionally determining an RDA score based on a combination of the values of the features.

主权项

1. A method for performing an RNA Disruption Assay (RDA) for cellular RNA optionally in response to a cytotoxic treatment optionally a treatment for a proliferative disorder optionally a cancer treatment, optionally chemotherapy, adjuvant or radiation treatment, wherein the method comprises:
obtaining at least one electropherogram dataset corresponding to a unique biological sample comprising cellular RNA optionally at a time point before, during or after the treatment; determining values for features from the at least one electropherogram dataset by using two identifying ranges to accommodate possible shifting of 18S and 28S peaks, detecting the 18S and 28S peaks, and calculating the features derived at least in part based on the located 18S and 28S peaks; and optionally determining an RDA score based on a combination of the values of the features.