D-Glucaric Acid Producing Bacterium, and Method for Manufacturing D-Glucaric Acid

摘要

The present invention provides a D-glucaric acid-producing bacterium and a method for producing D-glucaric acid. The present invention is characterized in that D-glucaric acid or a salt thereof is produced from one or more saccharides selected from the group consisting of D-glucose, D-gluconic acid and D-glucuronic acid with catalytic action of a specific alcohol dehydrogenase PQQ-ADH (1) and a specific aldehyde dehydrogenase PQQ-ALDH (2), and that D-glucaric acid or a salt thereof is produced by using a microorganism having the PQQ-ADH (1) and the PQQ-ALDH (2) or a processed product thereof in the presence of the one or more saccharides. The present invention can provide a microorganism having improved productivity of D-glucaric acid to be used for production of D-glucaric acid and a method for efficiently producing D-glucaric acid.

主权项

1. A microorganism belonging to the genus Pseudogluconobacter and having all the following characteristics:
(A) having activity of a specific alcohol dehydrogenase ADH (1) involved in production of D-glucaric acid; (B) having activity of a specific aldehyde dehydrogenase ALDH (2) involved in production of D-glucaric acid, the aldehyde dehydrogenase ALDH (2) being a pyrroloquinoline quinone-dependent enzyme having a molecular weight of 61,000±5,000 as measured by SDS-PAGE and a molecular weight of 180,000±10,000 as measured by gel filtration chromatography and having any of the following amino acid sequences (d) to (f): (d) an amino acid sequence represented by SEQ ID NO: 2; (e) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by substitution, deletion, insertion and/or addition of one or more amino acids; and (f) an amino acid sequence having 80% or higher homology with the amino acid sequence represented by SEQ ID NO: 2; and (C) having reduced or eliminated activity of aldehyde dehydrogenase ALDH (3) involved in production of keto acids.