METHOD FOR ENHANCED FERMENTATION THROUGH THE DESTRUCTION OF MITOCHONDRIAL DNA IN YEAST

摘要

Methods for enhanced yeast fermentation of plant material through the genetic modification of yeast comprising stably integrating into a yeast an inducible promoter operably linked to a mitochondrial targeting signal as well as at least one restriction enzyme and inducing the expression of the at least one restriction enzyme, wherein the restriction enzyme targets and destroys the mitochondrial DNA of the yeast. DNA constructs comprising at least an inducible promoter operably linked to a mitochondrial targeting signal as well as at least one restriction enzyme for induced expression of at least one restriction enzyme for destruction of mitochondrial DNA as well as transgenic yeast expressing a DNA construct for destruction of mitochondrial DNA and enhanced fermentation, are also disclosed.

主权项

1. A method for enhanced fermentation through the genetic modification of yeast comprising:
stably integrating into a yeast an inducible promoter coding sequence operably linked to a mitochondrial targeting signal coding sequence, wherein said mitochondrial targeting signal coding sequence is operably linked to at least one restriction enzyme coding sequence; inducing the expression of the at least one restriction enzyme, wherein said at least one restriction enzyme targets and destroys the mitochondrial DNA of the yeast; and inducing enhanced fermentation by said yeast.