Fluorescence detection device

摘要

To provide a fluorescence detection device that can effectively remove autofluorescence using a simple configuration. A fluorescence detection device comprises: semiconductor laser for emitting excitation light; an objective lens for converging excitation light onto a sample on a biosensor substrate; an anamorphic lens which introduces astigmatism to fluorescence that is from the biosensor substrate and incident on the objective lens and that passed through the objective lens; a spectral element for separating the fluorescence into a plurality of light rays; and a fluorescence detector for receiving the light rays separated by the spectral element. On the light receiving surface of the fluorescence detector, the spectral element splits the fluorescence so that the fluorescence generated at the sample is separated from the fluorescence generated at a specific depth position other than the sample position.

主权项

1. A fluorescence detection device that irradiates a sample holding carrier holding a fluorescently-labeled sample with irradiation light and detects fluorescence yielded from the sample irradiated with the irradiation light,
the fluorescence detection device comprising: a light source that emits the irradiation light; an objective lens that converge the irradiation light at the sample on the sample holding carrier; an astigmatism element for introducing astigmatism to fluorescence having entered the objective lens from the sample holding carrier and having passed through the objective lens; a spectral element for separating the fluorescence into a plurality of light rays; and a fluorescence detector for receiving the light ray separated by the spectral element, wherein the spectral element disperses the fluorescence on a light receiving surface of the fluorescence detector so that fluorescence generated from the sample position is separated from fluorescence generated from a specific depth position other than the sample position, and the fluorescence detector has a light-receiving section in a region in which the fluorescence generated from the sample position is irradiated and the fluorescence generated from the specific depth region other than the sample position is not irradiated.