发明名称 |
Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof |
摘要 |
The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils. |
申请公布号 |
US9045785(B2) |
申请公布日期 |
2015.06.02 |
申请号 |
US201213554975 |
申请日期 |
2012.07.20 |
申请人 |
DSM IP Assets B.V. |
发明人 |
Pfeifer, III Joseph W.;Hansen Jon Milton;Garcia Jose R.;Dong Xiao Daniel;Behrens Paul Warren;Apt Kirk E. |
分类号 |
C12P7/64;C12R1/89;C12N1/12 |
主分类号 |
C12P7/64 |
代理机构 |
|
代理人 |
Chen Xi;McGarrah Shannon |
主权项 |
1. A method of making a biomass of a microorganism having fatty acids and a concentration of EPA, comprising:
(a) fermenting the microorganism in a fermentor vessel to produce a fermentation broth having an aqueous phase and biomass wherein the aqueous phase has a dissolved gas wherein the microorganism comprises a Thraustochytrid that produces a biomass having at least 3% EPA of the total weight of the fatty acids; and (b) attaining a desired EPA level in the biomass by (i) adjusting the dissolved CO2 to >2% of the dissolved gas, (ii) optionally adjusting the pressure on the biomass, and (iii) optionally adjusting the temperature in the broth. |
地址 |
Heerlen NL |